白三烯（Leukotriene, LT）是花生四烯酸的5-脂氧合酶代謝的主要產物，能夠刺激白細胞的功能，包括溶酶體酶的釋放、粘附和多形核白細胞（即嗜中性、嗜堿性和嗜酸性白細胞）的聚集，被認為是炎癥性疾病和免疫調節(jié)的強效介質。半胱氨酰白三烯（LTC4、LTD4和LTE4）是通過將半胱氨酸衍生物加到LTA4上形成的，LTC4合酶將LTA4轉化為LTC4限制了半胱氨酰白三烯的形成速率。半胱氨酰白三烯作為炎癥中的強效脂質介質，可誘導平滑肌收縮和毛細血管通透性增加。

Enzo Life Sciences的高品質熱銷產品LTB4 ELISA kit（貨號：ADI-900-068）具有超高靈敏度和高文獻引用率，批次間差異小，上市多年廣受用戶好評。

產品亮點：

● 超靈敏度，對LTB4的超靈敏測量，檢測濃度低至5.63 pg/mL；

● 高文獻引用，在眾多同行評審的出版物中被廣泛引用；

● 寬動態(tài)范圍，適用于多種樣本類型；

● 高通量格式，做復孔的情況下，可在4小時內獲得多達39份樣本的結果；

● 批間批內差異小，檢測結果具有高度的可重復性；

● 完-全定量結果，優(yōu)于WB的半定量分析。

產品參數：

高分文獻引用：

近期，Adams發(fā)表了題為《Leukotriene A4 hydrolase inhibition improves age-related cognitive decline via modulation of synaptic function》的文章，在該項研究中，Adams等人使用了Enzo的LTB4 ELISA試劑盒檢測小鼠血漿，測定了老年小鼠、給藥白三烯A4水解酶抑制劑（LTA4H）的小鼠體內炎癥介質白三烯B4（LTB4）水平，證明了LTA4H抑制劑可以減少LTB4的產生，并改善小鼠與年齡相關的認知能力下降。

(A) Plasma LTB4 measured from unstimulated blood is significantly increased from 3 to 22.5 months (M). Unpaired t test, **P = 0.0011, F = 2.952 (n = 15 and 11). (B) Plasma LTB4 levels from calcimycin-stimulated blood are significantly reduced following 4 weeks of dosing with LTA4H inhibitors AKST1220 or SC57461A. Kruskal-Wallis test, P = 0.0006 followed by Dunn’s multiple comparisons: vehicle versus AKST1220, **P = 0.0034; vehicle versus SC57461A, *P = 0.0212; and AKST1220 versus SC57461A, P > 0.9999 (n = 5, 6, and 9).

Adams團隊對LTB4 ELISA kit的真實評價：

Q: Why did you use Enzo’s LTB4 ELISA Kit?

A: Species-independent measurement of LTB4 in culture supernatant, plasma, saliva, urine, and other cited sample types.

Q: How do you position Enzo’s LTB4 ELISA Kit?

A: The LTB4 ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. It is designed to assay up to 39 samples in duplicate with a broad dynamic range that is suitable for a wide variety of sample matrices, including bronchioalveolar lavage fluid, cell lysate, crevicular fluid, tissue, serum and whole blood.

LTB4 ELISA kit最新引用文獻：

1.  Leukotriene A4 hydrolase inhibition improves agerelated cognitive decline via modulation of synaptic function: J.M. Adams, et al.; Sci. Adv. 9, eadf8764 (2023), Abstract

2. Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract — Full Text

3.  Neobavaisoflavone inhibits allergic inflammatory responses by suppressing mast cell activation: R. Liang, et al.; Int. Immunopharmacol. 110, 108953 (2022), Abstract

4. Effect of Dysglycemia on Urinary Lipid Mediator Profiles in Persons With Pulmonary Tuberculosis: M.B. Arriaga, et al.; Front. Immunol. 13, 919802 (2022), Abstract

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